Geoarchaeological evidence of Holocene human impact and soil erosion on a till plain in Vorpommern (Kühlenhagen, NE-Germany)

Geoarchäologische Untersuchungen im Bereich eines glazigenen Solls verweisen auf den holozänen menschlichen Einfluss auf einer Grundmoränenplatte in Vorpommern in prähistorischer und historischer Zeit sowie dessen geomorphologische Rückkopplung. Die basale sedimentäre Füllung der untersuchten Depression besteht aus Torf, Mudde und Verlandungstorf und spiegelt früh- bis mittelholozäne hydrologische Schwankungen wider. Die obere Sequenz von wechselnden Kolluvien und organogenen Schichten deutet auf einen variierenden menschlichen Einfluss im Laufe der Zeit hin. Absolute AMS 14C-Datierungen von begrabenen Torfen und Mineralbodenhorizonten mit zum Teil erhöhten Anteilen organischer Substanz, abgesichert durch palynologische und archäologische Daten, belegen zwischenzeitliche Phasen von Oberflächenstabilität. Hingegen reflektieren die kolluvialen Sande Phasen erhöhter prähistorischer und historischer Besiedlung und Landnutzung. Die Sande können zum einen relativ durch die organogenen Schichten datiert und zum anderen zu den benachbarten archäologischen Funden in Beziehung gebracht werden. Erhöhte Erosionsprozesse werden demnach in das späte Neolithikum, den Übergang späte Bronzezeit zur frühen Eisenzeit, das Mittelalter und letztendlich in die Moderne gestellt. Die Akkumulation des jüngsten Kolluviums führt im Randbereich des Solls zur völligen Verfüllung bis an die rezente Oberfläche.researc

Partitioning a call graph

Splitting a large software system into smaller and more manageable units has become an important problem for many organizations. The basic structure of a software system is given by a directed graph with vertices representing the programs of the system and arcs representing calls from one program to another. Generating a good partitioning into smaller modules becomes a minimization problem for the number of programs being called by external programs. First, we formulate an equivalent integer linear programming problem with 0–1 variables. theoretically, with this approach the problem can be solved to optimality, but this becomes very costly with increasing size of the software system. Second, we formulate the problem as a hypergraph partitioning problem. This is a heuristic method using a multilevel strategy, but it turns out to be very fast and to deliver solutions that are close to optimal

Late Quaternary evolution of rivers, lakes and peatlands in northeast Germany reflecting past climatic and human impact – an overview

Die Kenntnis der regionalen Paläohydrologie ist eine wesentliche Grundlage für das Verständnis aktueller Umweltfragen, wie zum Beispiel nach den Gründen von hydrologischen Veränderungen, dem Einfluss von Landnutzungsstrategien und der Wirksamkeit von Renaturierungsvorhaben in Feuchtgebieten. Auch die Interpretation von Modellierungsergebnissen zu den künftigen Einflüssen des Klima- und Landnutzungswandels auf das Gewässersystem kann durch die Einbeziehung (prä-) historischer Analogien verbessert werden. Für das glazial geprägte nordostdeutsche Tiefland wurde eine Übersicht der vorliegenden paläohydrologischen Befunde für den Zeitraum der letzten etwa 20.000 Jahre erarbeitet. Die Entwicklung der Flüsse wurde mit Blick auf die Tal-/Auengenese und das Ablagerungsmilieu, die Veränderung des Tal- und Gerinneverlaufs sowie den Paläoabfluss bzw. das Paläohochwasser betrachtet. Wesentliche genetische Unterschiede bestehen zwischen Alt- (Elster- und Saalekaltzeit) und Jungmoränengebieten (Weichselkaltzeit) sowie zwischen hoch und tief gelegenen Tälern. Letztere sind stark durch Wasserspiegelveränderungen in der Nord- und Ostsee beeinflusst worden. Die Entwicklung der Seen wurde hinsichtlich der Seebildung, die überwiegend eine Folge der spätpleistozänen bis frühholozänen Toteistieftau-Dynamik ist, und der Veränderungen im Ablagerungsmilieu analysiert. Weiterhin standen Seespiegelveränderungen im Fokus, wobei sich hoch variable lokale Befunde mit einigen Übereinstimmungen zeigten. Der Überblick zur Moorentwicklung konzentrierte sich auf hydrogenetische Moorentwicklungsphasen und auf die langfristige Entwicklung des Grundwasserspiegels. Enge Beziehungen zwischen der Entwicklung der Flüsse, Seen und Moore bestanden insbesondere im Spätholozän durch komplexe Vermoorungsprozesse in den großen Flusstälern. Bis in das Spätholozän wurde die regionale Hydrologie überwiegend durch klimatische, geomorphologische und nicht-anthropogene biologische Faktoren gesteuert. Seit dem Spätmittelalter wurde in der Region das Gewässernetz und der Wasserkreislauf im starken Maß durch anthropogene Interventionen beeinflusst (z.B. Aufstau von Flüssen und Seen, Bau von Kanälen und Deichen, Moorkultivierung). In den letzten etwa 50 Jahren haben dann sogar die kurzfristigen anthropogenen Eingriffe, z.B. in Form von Abflussregulierung, Hydromelioration und künstlicher Seebildung, die Wirksamkeit langfristiger klimatischer und geomorphologischer Prozesse übertroffen.researc

Multiphoton In Vivo Microscopy of Embryonic Thrombopoiesis Reveals the Generation of Platelets through Budding.

Platelets are generated by specialized cells called megakaryocytes (MKs). However, MK's origin and platelet release mode have remained incompletely understood. Here, we established direct visualization of embryonic thrombopoiesis in vivo by combining multiphoton intravital microscopy (MP-IVM) with a fluorescence switch reporter mouse model under control of the platelet factor 4 promoter (Pf4CreRosa26mTmG). Using this microscopy tool, we discovered that fetal liver MKs provide higher thrombopoietic activity than yolk sac MKs. Mechanistically, fetal platelets were released from MKs either by membrane buds or the formation of proplatelets, with the former constituting the key process. In E14.5 c-Myb-deficient embryos that lack definitive hematopoiesis, MK and platelet numbers were similar to wild-type embryos, indicating the independence of embryonic thrombopoiesis from definitive hematopoiesis at this stage of development. In summary, our novel MP-IVM protocol allows the characterization of thrombopoiesis with high spatio-temporal resolution in the mouse embryo and has identified membrane budding as the main mechanism of fetal platelet production

Synthetic biology identifies the minimal gene set required for paclitaxel biosynthesis in a plant chassis

The diterpenoid paclitaxel (Taxol) is a chemotherapy medication widely used as a first-line treatment against several types of solid cancers. The supply of paclitaxel from natural sources is limited. However, missing knowledge about the genes involved in several specific metabolic steps of paclitaxel biosynthesis has rendered it difficult to engineer the full pathway. In this study, we used a combination of transcriptomics, cell biology, metabolomics, and pathway reconstitution to identify the complete gene set required for the heterologous production of paclitaxel. We identified the missing steps from the current model of paclitaxel biosynthesis and confirmed the activity of most of the missing enzymes via heterologous expression in Nicotiana benthamiana. Notably, we identified a new C4β-C20 epoxidase that could overcome the first bottleneck of metabolic engineering. We used both previously characterized and newly identified oxomutases/epoxidases, taxane 1β-hydroxylase, taxane 9α-hydroxylase, taxane 9α-dioxygenase, and phenylalanine-CoA ligase, to successfully biosynthesize the key intermediate baccatin III and to convert baccatin III into paclitaxel in N. benthamiana. In combination, these approaches establish a metabolic route to taxoid biosynthesis and provide insights into the unique chemistry that plants use to generate complex bioactive metabolites

Early treatment of complex located pediatric low-grade gliomas using iodine-125 brachytherapy alone or in combination with microsurgery

To analyze efficacy, functional outcome, and treatment toxicity of low-dose rate I-125 brachytherapy (SBT) alone or in combination with best safe resection (in case of larger tumor volumes) as first-line treatment for pediatric low-grade gliomas (PLGGs) not suitable for complete resection. Consecutively treated (2000-2014) complex located circumscribed WHO grade I/II PLGGs were included. For small tumors (4cm in diameter) SBT alone was performed;for larger tumors best safe resection and subsequent SBT was chosen. Temporary Iodine-125 seeds were used (median reference dose: 54Gy). Treatment response was estimated with the modified MacDonald criteria. Analysis of functional outcome included ophthalmological, endocrinological and neurological evaluation. Survival was analyzed with the Kaplan-Meier method. Prognostic factors were obtained from proportional hazards models. Toxicity was categorized according to the Common Terminology Criteria for Adverse Events. Fifty-eight patients were included treated either with SBT alone (n=39) or with SBT plus microsurgery (n=19). Five-year progression-free survival was 87%. Two patients had died due to tumor progression. Among survivors, improvement/stabilization/deterioration of functional deficits was seen in 20/14/5 patients, respectively. Complete/partial response had beneficial impact on functional scores (P=0.02). The 5-year estimated risk to receive adjuvant radiotherapy/chemotherapy was 5.2%. The overall early (delayed) toxicity rate was 8.6% (10.3%), respectively. No permanent morbidity occurred. In complex located PLGGs, early SBT alone or combined with best safe resection preserves/improves functional scores and results in tumor control rates usually achieved with complete resection. Long-term analysis is necessary for confirmation of these results

Assessment of intraductal carcinoma in situ (DCIS) using grating-based X-ray phase-contrast CT at conventional X-ray sources: An experimental ex-vivo study

Background The extent of intraductal carcinoma in situ (DCIS) is commonly underestimated due to the discontinuous growth and lack of microcalcifications. Specimen radiography has been established to reduce the rate of re-excision. However, the predictive value for margin assessment with conventional specimen radiography for DCIS is low. In this study we assessed the potential of grating-based phase-contrast computed tomography (GBPC-CT) at conventional X-ray sources for specimen tomography of DCIS containing samples. Materials and methods GBPC-CT was performed on four ex-vivo breast specimens containing DCIS and invasive carcinoma of non-specific type. Phase-contrast and absorption-based datasets were manually matched with corresponding histological slices as the standard of reference. Results Matching of CT images and histology was successful. GBPC-CT showed an improved soft tissue contrast compared to absorption-based images revealing more histological details in the same sections. Non-calcifying DCIS exceeding the invasive tumor could be correlated to areas of dilated bright ducts around the tumor. Conclusions GBPC-CT imaging at conventional X-ray sources offers improved depiction quality for the imaging of breast tissue samples compared to absorption-based imaging, allows the identification of diagnostically relevant tissue details, and provides full three-dimensional assessment of sample margins

Experimental Realisation of High-sensitivity Laboratory X-ray Grating-based Phase-contrast Computed Tomography

The possibility to perform high-sensitivity X-ray phase-contrast imaging with laboratory grating-based phase-contrast computed tomography (gbPC-CT) setups is of great interest for a broad range of high-resolution biomedical applications. However, achieving high sensitivity with laboratory gbPC-CT setups still poses a challenge because several factors such as the reduced flux, the polychromaticity of the spectrum, and the limited coherence of the X-ray source reduce the performance of laboratory gbPC-CT in comparison to gbPC-CT at synchrotron facilities. In this work, we present our laboratory X-ray Talbot-Lau interferometry setup operating at 40 kVp and describe how we achieve the high sensitivity yet unrivalled by any other laboratory X-ray phase-contrast technique. We provide the angular sensitivity expressed via the minimum resolvable refraction angle both in theory and experiment, and compare our data with other differential phase-contrast setups. Furthermore, we show that the good stability of our high-sensitivity setup allows for tomographic scans, by which even the electron density can be retrieved quantitatively as has been demonstrated in several preclinical studies